wizard promega genomic dna extraction kit Search Results


wizard genomic dna purification kit 12  (Promega)
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Promega wizard genomic dna purification kit 12
Wizard Genomic Dna Purification Kit 12, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wizard genomic dna purification kit 12 - by Bioz Stars, 2026-10
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wizard ® genomic dna purification a1120  (Promega)
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Promega wizard ® genomic dna purification a1120
DNA extraction protocols tested on five VPRI apple powdery mildew P . leucotricha specimens in this study.
Wizard ® Genomic Dna Purification A1120, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wizard ® genomic dna purification a1120 - by Bioz Stars, 2026-10
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wizard sv gel and pcr clean-up system  (Promega)
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Promega wizard sv gel and pcr clean-up system
DNA extraction protocols tested on five VPRI apple powdery mildew P . leucotricha specimens in this study.
Wizard Sv Gel And Pcr Clean Up System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wizard sv gel and pcr clean-up system - by Bioz Stars, 2026-10
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genomic dna human blood  (Promega)
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Promega genomic dna human blood
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Genomic Dna Human Blood, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wizard+promega+genomic+dna+extraction+kit/wizard+genomic+blood+dna+isolation+kit/pmc02262070-202-0-5
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genomic dna human blood - by Bioz Stars, 2026-10
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genome dna extraction kit  (Promega)
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Promega genome dna extraction kit
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Genome Dna Extraction Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wizard+promega+genomic+dna+extraction+kit/genome+dna+extraction+kit/pm21161889-41-10-14
Average 90 stars, based on 1 article reviews
genome dna extraction kit - by Bioz Stars, 2026-10
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wizard dna isolation  (Promega)
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Promega wizard dna isolation
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Wizard Dna Isolation, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wizard+promega+genomic+dna+extraction+kit/wizard++genomic+dna+purification+kit/us10618933-536-18-20
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wizard dna isolation - by Bioz Stars, 2026-10
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cell lysis solution wizard genomic dna purification kit  (Promega)
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Promega cell lysis solution wizard genomic dna purification kit
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Cell Lysis Solution Wizard Genomic Dna Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wizard+promega+genomic+dna+extraction+kit/cell+lysis+solution+wizard+genomic+dna+purification+kit/pmc12041197-260-28-33
Average 90 stars, based on 1 article reviews
cell lysis solution wizard genomic dna purification kit - by Bioz Stars, 2026-10
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human genomic dna extraction kit  (Promega)
90
Promega human genomic dna extraction kit
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Human Genomic Dna Extraction Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wizard+promega+genomic+dna+extraction+kit/human+genomic+dna+extraction+kit/pmc04620187-204-6-10
Average 90 stars, based on 1 article reviews
human genomic dna extraction kit - by Bioz Stars, 2026-10
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wizard genomic dna purification kit # 1120  (Promega)
90
Promega wizard genomic dna purification kit # 1120
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Wizard Genomic Dna Purification Kit # 1120, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wizard+promega+genomic+dna+extraction+kit/wizard+genomic+dna+purification+kit+++1120/pmc11298459-138-10-17
Average 90 stars, based on 1 article reviews
wizard genomic dna purification kit # 1120 - by Bioz Stars, 2026-10
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genomic dnas promega  (Promega)
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Promega genomic dnas promega
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Genomic Dnas Promega, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wizard genomic dna purification kit promega kit  (Promega)
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Promega wizard genomic dna purification kit promega kit
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Wizard Genomic Dna Purification Kit Promega Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wizard+promega+genomic+dna+extraction+kit/wizard+tm+genomic+dna+purification+kit+promega+h/10__3390_slash_catal10060651-223-27-32
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wizard genomic dna purification kit promega kit - by Bioz Stars, 2026-10
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cell genomic dna purification kit  (Promega)
90
Promega cell genomic dna purification kit
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Cell Genomic Dna Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wizard+promega+genomic+dna+extraction+kit/wizard+genomic+dna+purification+kit+cultured+cells/10__1016_slash_j__aqrep__2021__100899-50-10-15
Average 90 stars, based on 1 article reviews
cell genomic dna purification kit - by Bioz Stars, 2026-10
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Image Search Results


DNA extraction protocols tested on five VPRI apple powdery mildew P . leucotricha specimens in this study.

Journal: PLoS ONE

Article Title: Rediscovering an old foe: Optimised molecular methods for DNA extraction and sequencing applications for fungarium specimens of powdery mildew (Erysiphales)

doi: 10.1371/journal.pone.0232535

Figure Lengend Snippet: DNA extraction protocols tested on five VPRI apple powdery mildew P . leucotricha specimens in this study.

Article Snippet: Wizard ® Genomic DNA Purification , WizG , Promega (A1120) , Precipitation.

Techniques: DNA Extraction, Extraction, Binding Assay, Purification, Lysis, DNA Purification

Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell genomic DNA. C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.

Journal: BMC Genomics

Article Title: Human glycolipid transfer protein ( GLTP ) genes: organization, transcriptional status and evolution

doi: 10.1186/1471-2164-9-72

Figure Lengend Snippet: Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell genomic DNA. C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.

Article Snippet: Genomic DNA from human blood (Promega) or isolated from various cell lines (Sigma-Aldrich, GenElute™ mammalian genomic DNA miniprep kit) was bisulfite converted using manufacturer's recommendations (CpGenome Fast; Chemicon, Temecula, CA).

Techniques: Methylation, Clone Assay, Reverse Transcription Polymerase Chain Reaction, Combined Bisulfite Restriction Analysis Assay, Agarose Gel Electrophoresis, Amplification, Sequencing, Negative Control, Molecular Weight